Calculating rabies virus neutralizing antibodies titres by flow cytometry

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Calculating rabies virus neutralizing antibodies titres by flow cytometry.

The determination of the rabies neutralizing antibody (VNA) response after immunization against rabies is an acceptable index of the efficacy of a vaccine and a successful treatment. Several tests have been developed in attempt to improve the assessment of VNA, from mice inoculation to cell-culture fluorescence inhibition tests. All of them, however, present special difficulties in terms of rea...

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Rabbit anti-idiotypic antibodies (alpha Id Ab) were prepared against five murine monoclonal antibodies (mAb) specific for the rabies virus glycoprotein. Four of the mAb were directed against three known, type-specific, neutralizing sites on the glycoprotein, and the other mAb was directed against a topographically uncharacterized, nonneutralizing epitope. An absence of significant cross-reactiv...

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Antibody response in vitro to an animal virus: production of rabies virus neutralizing antibodies by mouse cells in culture.

Rabies virus neutralizing antibodies were produced in vitro by the exposure of mouse spleen cells to live and inactivated rabies virus suspensions and to sheep erythrocytes coated with rabies virus. These antibodies did not neutralize two other rhabdoviruses: Kern Canyon and vesicular stomatitis viruses, and were precipitable by treatment with an antiserum to mouse IgG. Removal of "glass-adheri...

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Presence of Virus Neutralizing Antibodies in Cerebral Spinal Fluid Correlates with Non-Lethal Rabies in Dogs

BACKGROUND Rabies is traditionally considered a uniformly fatal disease after onset of clinical manifestations. However, increasing evidence indicates that non-lethal infection as well as recovery from flaccid paralysis and encephalitis occurs in laboratory animals as well as humans. METHODOLOGY/PRINCIPAL FINDINGS Non-lethal rabies infection in dogs experimentally infected with wild type dog ...

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BACKGROUND Animal control measures in Latin America have decreased the incidence of urban human rabies transmitted by dogs and cats; currently most cases of human rabies are transmitted by bats. In 2004-2005, rabies outbreaks in populations living in rural Brazil prompted widespread vaccination of exposed and at-risk populations. More than 3,500 inhabitants of Augusto Correa (Pará State) receiv...

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ژورنال

عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo

سال: 2002

ISSN: 0036-4665

DOI: 10.1590/s0036-46652002000300007